Background<\/strong><\/p>\nHistaminolytic activity mediated by diamine oxidase (DAO) is present in plasma after induction of severe anaphylaxis in rats, guinea pigs, and rabbits. Heparin released during mast cell degranulation in the gastrointestinal tract might liberate DAO from heparin\u2010sensitive storage sites. DAO release during anaphylaxis has not been demonstrated in humans.<\/p>\n
Methods<\/strong><\/p>\nPlasma DAO, tryptase, and histamine concentrations of four severe anaphylaxis events were determined at multiple serial time points in two patients with systemic mastocytosis. The histamine degradation rates were measured in anaphylaxis samples and in pregnancy sera and plasma with comparable DAO concentrations.<\/p>\n
Results<\/strong><\/p>\nMean DAO (132\u00a0ng\/mL) and tryptase (304\u00a0ng\/mL) concentrations increased 187\u2010 and 4.0\u2010fold, respectively, over baseline values (DAO 0.7\u00a0ng\/mL, tryptase 76\u00a0ng\/mL) during severe anaphylaxis. Under non\u2010anaphylaxis conditions, DAO concentrations were not elevated in 29 mastocytosis patients compared to healthy volunteers and there was no correlation between DAO and tryptase levels in mastocytosis patients. The histamine degradation rate of DAO in plasma from mastocytosis patients during anaphylaxis is severely compromised compared to DAO from pregnancy samples.<\/p>\n
Conclusion<\/strong><\/p>\nDuring severe anaphylaxis in mastocytosis patients, DAO is likely released from heparin\u2010sensitive gastrointestinal storage sites. The measured concentrations can degrade histamine, but DAO activity is compromised compared to pregnancy samples. For accurate histamine measurements during anaphylaxis, DAO inhibition is essential to inhibit further histamine degradation after blood withdrawal. Determination of DAO antigen levels might be of clinical value to improve the diagnosis of mast cell activation.<\/p>\n
<\/p>\n","protected":false},"excerpt":{"rendered":"
Massive release of the histamine\u2010degrading enzyme diamine oxidase during severe anaphylaxis in\u00a0mastocytosis\u00a0patients Download pdf Thomas Boehm,\u00a01\u00a0Birgit Reiter,\u00a02\u00a0Robin Ristl,\u00a03\u00a0Karin Petroczi,\u00a01\u00a0Wolfgang Sperr,\u00a04\u00a0Thomas Stimpfl,\u00a02\u00a0Peter Valent,\u00a04\u00a0and\u00a0Bernd Jilma\u00a01 Allergy. 2019 Mar; 74(3): 583\u2013593. Published online […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[111,119],"tags":[213,262,135,149],"class_list":["post-10696","post","type-post","status-publish","format-standard","hentry","category-dao-and-inmunological-symptoms","category-scientific-publications","tag-dao-activity","tag-dao-and-anaphylaxis-in-mastocytosis","tag-diamine-oxidase","tag-histamine"],"yoast_head":"\n
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